Molecular Machines & Industries

#CellCutFact No 2: Fully control your cell lysis conditions for reliable RNAseq

The MMI CellCut offers a unique and patented way (US Patent #8,945,866) to isolate dissected tissue so that lysis buffer is added only after all cells are collected. Other systems require lysis buffer in the isolation tube to be able to collect cells. Since the isolation procedure will take some time depending on the amount […]

New #CellCutFacts series starts today!

Today, we will start a new series to inform you about helpful and unique features of our MMI CellCut Laser Microdissection system which we name #CellCutFact. From now on, we will post one #CellCutFact every week in our MMI blog, on LinkedIn and on Twitter. Connect with us and stay tuned to not miss any […]

Identifying molecular motifs of fibrosing pulmonary diseases using Laser Microdissection

Interstitial lung disease (ILD) is an umbrella for a multitude of different diseases, such as idiopathic pulmonary fibrosis (IPF), characterized by a typical usual interstitial pneumonia (UIP) pattern, nonspecific interstitial pneumonia (NSIP), organizing pneumonia (OP) and alveolar fibroelastosis (AFE). For diagnosis, currently employed morphological criteria are not reliably discriminating different ILDs. Therefore, Danny Jonigk and […]

Isolating single cells for omics analysis

You want to isolate a single living cell for subsequent molecular analysis, such as transcriptomics or proteomics? What are you waiting for? The MMI CellCut allows you to cut single cells without harming them. The cells (grown in special 18-well Membrane Slides) are still covered with medium during cutting, they even can be kept in […]

Does surgery contribute to development of circulating tumor cells and metastases?

Initial breast cancer therapies aim at eliminating the main cancer burden by removal of the primary tumor. However, the most life-threatening aspect is not its growth but rather the capacity of the primary tumor to release cells into the blood circulation, which then can settle in vital organs and grow into metastases, impairing the function […]

Developmental biology in 4D

In developmental biology, many processes are not yet fully understood as the regulatory network and interplay of development-related transcription factors is very complex. The study published by Cui et al. incorporated time as an additional dimension to 3D Geo-Seq data to uncover transcription factor activity at key developmental timepoints in germ layer specification and lineage […]